Biochar-immobilized Bacillus spp. for heavy metals bioremediation: A review on immobilization techniques, bioremediation mechanisms and effects on soil.

Heavy metals contamination present risks to ecosystems and human health. Bioremediation is a technology that has been applied to minimize the levels of heavy metals contamination. However, the efficiency of this process varies according to several biotic and abiotic aspects, especially in environments with high concentrations of heavy metals. Therefore, microorganisms immobilization in different materials, such as biochar, emerges as an alternative to alleviate the stress that heavy metals have on microorganisms and thus improve the bioremediation efficiency. In this context, this review aimed to compile recent advances in the use of biochar as a carrier of bacteria, specifically Bacillus spp., with subsequent application for the bioremediation of soil contaminated with heavy metals. We present three different techniques to immobilize Bacillus spp. on biochar. Bacillus strains are capable of reducing the toxicity and bioavailability of metals, while biochar is a material that serves as a shelter for microorganisms and also contributes to bioremediation through the adsorption of contaminants. Thus, there is a synergistic effect between Bacillus spp. and biochar for the heavy metals bioremediation. Biomineralization, biosorption, bioreduction, bioaccumulation and adsorption are the mechanisms involved in this process. The application of biochar-immobilized Bacillus strains results in beneficial effects on the contaminated soil, such as the reduction of toxicity and accumulation of metals in plants, favoring their growth, in addition to increasing microbial and enzymatic activity in soil. However, competition and reduction of microbial diversity and the toxic characteristics of biochar are reported as negative impacts of this strategy. More studies using this emerging technology are essential to improve its efficiency, to elucidate the mechanisms and to balance positive and negative impacts, especially at the field scale.

A review of the main methods for composite adsorbents characterization.

Adsorption is a promising technology for removing several contaminants from aqueous matrices. In the last years, researchers worldwide have been working on developing composite adsorbents to overcome some limitations and drawbacks of conventional adsorbent materials, which depend on various factors, including the characteristics of the adsorbents. Therefore, it is essential to characterize the composite adsorbents to describe their properties and structure and elucidate the mechanisms, behavior, and phenomenons during the adsorption process. In this sense, this work aimed to review the main methods used for composite adsorbent characterization, providing valuable information on the importance of these techniques in developing new adsorbents. In this paper, we reviewed the following methods: X-Ray diffraction (XRD); spectroscopy; scanning electron microscopy (SEM); N2 adsorption/desorption isotherms (BET and BJH methods); thermogravimetry (TGA); point of zero charge (pHPZC); elemental analysis; proximate analysis; swelling and water retention capacities; desorption and reuse.

Rhodococcus: A promising genus of actinomycetes for the bioremediation of organic and inorganic contaminants.

Rhodococcus is a genus of actinomycetes that has been explored by the scientific community for different purposes, especially for bioremediation uses. However, the mechanisms governing Rhodococcus-mediated bioremediation processes are far from being fully elucidated. In this sense, this work aimed to compile the recent advances in the use of Rhodococcus for the bioremediation of organic and inorganic contaminants present in different environmental compartments. We reviewed the bioremediation capacity and mechanisms of Rhodococcus spp. in the treatment of polycyclic aromatic hydrocarbons, phenolic substances, emerging contaminants, heavy metals, and dyes given their human health risks and environmental concern. Different bioremediation techniques were discussed, including experimental conditions, treatment efficiencies, mechanisms, and degradation pathways. The use of Rhodococcus strains in the bioremediation of several compounds is a promising approach due to their features, primarily the presence of appropriate enzyme systems, which result in high decontamination efficiencies; but that vary according to experimental conditions. Besides, the genus Rhodococcus contains a small number of opportunistic species and pathogens, representing an advantage from the point of view of safety. Advances in analytical detection techniques and Molecular Biology have been collaborating to improve the understanding of the mechanisms and pathways involved in bioremediation processes. In the context of using Rhodococcus spp. as bioremediation agents, there is a need for more studies that 1) evaluate the role of these actinomycetes on a pilot and field scale; 2) use genetic engineering tools and consortia with other microorganisms to improve the bioremediation efficiency; and 3) isolate new Rhodococcus strains from environments with extreme and/or contaminated conditions aiming to explore their adaptive capabilities for bioremediation purposes.

Use of soil actinomycetes for pharmaceutical, food, agricultural, and environmental purposes.

In this article, we reviewed the international scientific production of the last years on actinomycetes isolated from soil aiming to report recent advances in using these microorganisms for different applications. The most promising genera, isolation conditions and procedures, pH, temperature, and NaCl tolerance of these bacteria were reported. Based on the content analysis of the articles, most studies have focused on the isolation and taxonomic description of new species of actinomycetes. Regarding the applications, the antimicrobial potential (antibacterial and antifungal) prevailed among the articles, followed by the production of enzymes (cellulases and chitinases, etc.), agricultural uses (plant growth promotion and phytopathogen control), bioremediation (organic and inorganic contaminants), among others. Furthermore, a wide range of growth capacity was verified, including temperatures from 4 to 60 °C (optimum: 28 °C), pH from 3 to 13 (optimum: 7), and NaCl tolerance up to 32% (optimum: 0-1%), which evidence a great tolerance for actinomycetes cultivation. Streptomyces was the genus with the highest incidence among the soil actinomycetes and the most exploited for different uses. Besides, the interest in isolating actinomycetes from soils in extreme environments (Antarctica and deserts, for example) is growing to explore the adaptive capacities of new strains and the secondary metabolites produced by these microorganisms for different industrial interests, especially for pharmaceutical, food, agricultural, and environmental purposes.

Environmental assessment of viticulture waste valorisation through composting as a biofertilisation strategy for cereal and fruit crops.

Composting is a solid waste management alternative that avoids the emission of methane associated with its disposal in landfill and reduces or eliminates the need for chemical fertilisers if compost is applied. The main objective of this study was to analyse the environmental burdens of composting as a way to achieve a more circular valorisation of wine waste. To do so, with the purpose of identifying optimal operational conditions and determining the "hotspots" of the process, the life cycle assessment (LCA) methodology was used. The consumption of diesel fuel in machinery was determined to be the main critical point in the environmental effects of the system, followed by the transport and distribution of the compost. After the application of compost instead of mineral fertilisers, corn, tomato and strawberry crops would have a better environmental performance in most impact categories. In this sense, a maximum improvement of 65% in terrestrial ecotoxicity is achieved in strawberry cultivation. In light of the results obtained, it is demonstrated that composting is a suitable way of organic waste valorisation according to Circular Economy principles.